Expanded T-cell clones were most frequently CD8 + with some detectable in peripheral blood or normal kidney and others found exclusively within the tumor. TCR repertoire analysis also revealed increased clonal expansion in ccRCC tumors compared with non-clear cell histologies or normal tissues. Multiplex immunohistochemistry revealed that T-cell density was higher in clear cell RCC (ccRCC) than in other renal tumor histologies with spatially nonuniform T-cell hotspots and exclusion zones. To identify and further characterize tumor-associated, clonally expanded T cells, we characterized the density, spatial distribution, T-cell receptor (TCR) repertoire, and transcriptome of tumor-infiltrating T cells from 14 renal tumors at the time of resection and compared them with T cells in peripheral blood and normal adjacent kidney. Recent analyses associate clonally expanded T cells present in the tumor at diagnosis with responses to immune checkpoint inhibitors (ICIs). Objective responses of metastatic renal cell carcinoma (RCC) associated with systemic immunotherapies suggest the potential for T-cell-mediated tumor clearance. 3Department of Medicine, Division of Medical Oncology, University of Washington School of Medicine, Seattle, WA, United States.2Department of Laboratory Medicine and Pathology, University of Washington School of Medicine, Seattle, WA, United States.1Clinical Research Division, Fred Hutchinson Cancer Center, Seattle, WA, United States.
Towlerton 1 Shreeram Akilesh 1,2 Chris P.
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